Job Board > Postdoctoral Fellow - University of Manitoba
Drs El-Gabalawy and O’Neil are conducting a prospective clinical trial in Rheumatoid Arthritis prevention. The Research Associate position will be filled by an individual with a PhD in Immunology or related discipline, with a specific interest immunophenotyping lymphocytes using flow cytometry.
Prepare cryopreserved peripheral blood mononuclear cells (PBMC) from an interventional clinical trial for flow cytometry experiments.
Perform multi-colour flow cytometry, including cell staining and data acquisition (Beckman Coulter CytoFLEX).
Analyze data from flow cytometry experiments in conjunction with Immunology Core staff using FlowJo and other analytical pipelines.
Perform experiments on serum/plasma/PBMCs obtained from clinical trials using (but not restricted to): ELISA, RNA-seq, nanostring, real-time PCR, metabolomics.
Prepare the analyzed data into tables and figures suitable for publication.
Author the initial draft of manuscripts based on the acquired data.
PhD Immunology or Equivalent
Dr. Liam O’Neil is a member of the Manitoba Centre for Proteomics and Systems Biology, and his research focuses on understanding the biological events that occur prior to the development of rheumatoid arthritis.
He previously trained at the National Institutes of Health (Bethesda, MD) where he elucidated a mechanism linking neutrophil extracellular trap (NET) formation and autoantibody responses in rheumatoid arthritis.
His aim is to better understand the pre-clinical phase of rheumatoid arthritis, interface immunology and systems biology to elucidate mechanisms of rheumatoid arthritis pathogenesis.