Seeking an individual to join the lab of Dr. Sarah Crome within the Ajmera Transplant Centre at Toronto General Hospital Research Institute and the Department of Immunology at the University of Toronto. The research team is comprised of scientifically enthusiastic individuals who value diversity and support each other’s individual goals.
Key responsibilities of the Research Technician will include the following:
- Performing experiments that will include cell culture, lentiviral transductions, flow cytometry, and humanized animal models.
- Processing of patient samples including human tissues and blood.
- Preparing lentiviral vectors, maintaining molecular biology stocks, and performing cloning experiments
- Responsible for coordinating and performing assigned routine standard testing.
- Responsible for ensuring reagent supplies are replenished and coordinating lab ordering
- Ensuring cleaning procedures, calibrations and/or validation of equipment is performed regularly.
- Aid in generating SOPs, and maintaining accurate records of procedures, animals, and inventories.
- Attending and participating in laboratory meetings.
- Training or assisting graduate students in laboratory techniques as appropriate.
- Contribute to data analysis, as well as figure and manuscript preparations
• Minimum B.Sc plus experience in a research setting (3+ years). M.Sc or additional education in immunology, biological sciences or other related disciplines an asset.
• Experience in the following techniques is an asset: flow cytometry, tissue staining, humanized mouse models, processing patient samples (tissue and blood), working with lentivirus or other viral vectors, molecular cloning and good manufacturing practices.
• Self-motivated, reliable, and detail-oriented with excellent organizational skills.
• Demonstrated ability to work independently and willingness to work closely with other members of the team.
• Computer skills: Microsoft office proficiency, experience with Graphpad prism, Adobe illustrator, Flowjo or R an asset.
• Demonstrated strong written and verbal communication skills.
How to Apply:
Applications can be made via UHN careers website (https://www.recruitingsite.com/csbsites/uhncareers/careers.asp)
or by emailing applications directly to sarah.crome[at]uhnreserach.ca .
Applications should include the following:
1. A detailed CV
2. A cover letter outlining relevant experiences
3. Contact information for 2-3 references.
Review of applications will begin February 1, 2023
Salary: Dependent on prior experience
Sarah Crome, PhD
Scientist | University Health Network | Toronto General Hospital Research Institute | Ajmera Transplant Centre
Assistant Professor | University of Toronto | Temerty Faculty of Medicine | Department of Immunology
University Health Network (UHN) is a research hospital affiliated with the University of Toronto and a member of the Toronto Academic Health Science Network. The scope of research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. Research across UHN's five research institutes spans the full spectrum of diseases and disciplines, including cancer, cardiovascular sciences, transplantation, neural and sensory sciences, musculoskeletal health, rehabilitation sciences, and community and population health.
Dr. Crome’s research program is focused on identifying mechanisms of immune tolerance and developing immunotherapies for transplantation and autoimmunity. There is a particular interest in understanding the role of specialized tissue-resident immune cells in immune tolerance and developing ways to harness these lymphocytes in novel therapies.
More details on the research lab can be found at: